Motility Test Agar

Motility Test Agar (From Liofilchem)

Dehydrated medium for detection of motility of gram-negative enteric bacilli according to UNI EN ISO 11290-1:2005

Composition (g/l)
Casein Peptone – 20.0
Meat – 6.1
Agar – 3.5
Final pH – 7.2 ± 0.2

MOTILITY TEST AGAR is a dehydrated medium for detection of motility of gram-negative enteric bacilli according to UNI EN ISO
11290-1:2005
PRINCIPLE
Casein peptones and meat provide nitrogen, vitamins, minerals and amino acids essential for growth. Agar is the solidifying agent in a
low concentration to enable the motility to be seen.
PREPARATION
Suspend 29.6 g of powder in 1 litre of distilled or deionized water. Heat to boiling and shake until completely dissolved. Dispense in final
containers. Sterilise at 121°C for 15 minutes.
TECHNIQUE
Inoculate tubes with a pure culture by stabbing the center of the column of medium to greater than half the depth.
Incubate tubes for 24-48 hours at 36±1°C in an aerobic atmosphere.
INTERPRETATION OF RESULTS
Motility is evidenced by the presence of diffuse growth away from the line or spot of inoculation. Nonmotile organisms grow only along
the line of inoculation. Negative tubes can be reincubated at 25±2°C for an additional 5 days, if desired.
Consult appropriate texts for results with specific organisms2,3.
STORAGE AND TRANSPORT CONDITIONS
The powder is very hygroscopic: store the powder at 10-30°C, in a dry environment, in its original container tightly closed until the expiry
date on the label or until signs of deterioration or contamination are evident. Storage or transport at 2-10°C do not alter in any way the
performance of the product. Store prepared media at 2-8°C.
WARNING AND PRECAUTIONS
The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is
not classified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use.. The product is
designed for In vitro diagnostic use and must be used only by properly trained operators.
DISPOSAL OF WASTE
Disposal of waste must be carried out according to the national and local regulations in force.

Weight: 500g