GGT Clinical Chemistry Reagent

GGT Clinical Chemistry Reagent

Enzymatic colorimetric method
KINETIC

PRINCIPLE

Gamma-glutamyltransferase (γ-GT) catalyzes the transfer of a γ- glutamyl group from γ-glutamyl-3-carboxy-4-nitroanilide to glycylglycine with the formation of L-γ-glutamyl-glycylglycine and 5 amino-2-nitro-benzoate.

The amount of 5-amino-2-nitro-benzoate formed, monitored kinetically at 405 nm, is proportional to the enzyme activity present in the sample.

REAGENT COMPOSITION

4 x 100 ml
R1 Buffer/Glycylglycine. TRIS 133 mmol/L pH 8.2, glycylglycine
138 mmol/L, sodium azide 0.09%.
R2 Substrate/Glupa-C. L-γ-glutamyl-3-carboxy-4-nitroanilide
23 mmol/L, ethylene glycol 900 mmol/L, sodium azide
0.09%.

STORAGE AND STABILITY
Store at 2-8ºC.

REAGENT PREPARATION
Working reagent. Mix 4 mL of R1 + 1 mL of R2. Stable for 3 weeks at
2-8ºC or for 5 days at 15-25ºC.
Protect from light.

SAMPLES
Serum or EDTA plasma free of hemolysis. Fluoride, citrate and
oxalate inhibit γ-GT activity2
.
The enzyme in the sample is stable for at least 1 week at 2-8ºC and
for at least 2 months when frozen. Only freeze once. Discard contaminated specimens.
The samples should be handled as potentially infectious in accordance with Good Manufacturing Practices.

MATERIALS REQUIRED
− Photometer or spectrophotometer with cuvette thermostatic compartment set at 25/30/37ºC, capable of reading at 405 nm.
− Stopwatch, strip-chart recorder or printer.
− Cuvettes with 1-cm pathlength.
− Pipettes to measure reagent and samples.
− Ref. 1975005 Human Multicalibrator (optional).
− General laboratory equipment.

GGT Clinical Chemistry Reagent per  box