ALT/GPT Chemistry Reagents

ALT/GPT Chemistry Reagents

Brand: Cromatest

PRINCIPLE 

Alanine aminotransferase (ALT/GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate. The latter is reduced to lactate by  lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH).

The reaction is monitored kinetically at 340 nm by the rate of decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the activity of ALT present in the sample.

Product Overview

• IFCC standards: This test has been formulated according the standardized method
  described by IFCC. Clin Chem Lab Med 2002; 40(7) : 718-7242
• UV enzymatic method
• Kinetic
• ALT/GPT 3 x 100ml
• Storage: at 2-8ºC

REAGENT COMPOSITION

R1 ALT substrate: TRIS buffer 150 mmol/L pH 7.3, L-alanine
750 mmol/L, lactate dehydrogenase  1350 U/L.
R2 ALT coenzyme. NADH 1.3 mmol/L, 2-oxoglutarate 75
mmol/L. Biocides.

REAGENT PREPARATION
Working reagent. Mix 4 mL of R1 + 1 mL of R2. Stable for 4 weeks
at 2-8ºC. Protect from light.
SAMPLES
Serum, EDTA or heparinized plasma free of hemolysis.
ALT is stable in serum or plasma 24 hours at room temperature
and for 1 week at 2-8ºC.

MATERIALS REQUIRED

• Photometer or spectrophotometer with a thermostatic cell
  compartment set at 30/37ºC, capable of reading at 340 nm.
• Stopwatch, strip-chart recorder or printer.
• Cuvettes with 1-cm pathlength.
• Pipettes to measure reagent and samples.

ALT/GPT Chemistry Reagents per box