One Step HIV 1+2/Syphilis Combo Card Tests


If you have any questions about this product call us:
0535 106 338

 One Step HIV 1+2/Syphilis Combo Card Test

Brand:  First Response

Contains 50 test cards per box

HIV and Syphilis may have similarities in terms of symptoms.  For example, rash is a symptom of both HIV and Syphilis. This may create uncertainties regarding one´s health status, that is why the One Step HIV 1+2/Syphilis Combo Card Test is useful in differentiating between the two infections for the appropriate treatment intervention to made.

The One Step HIV 1+2/Syphilis Combo Card Test  is a rapid qualitative screening in vitro diagnostic test for the detection of antibodies (IgG & IgM) specific to HIV (type 1 & 2) and Treponema pallidum in human serum, plasma or venous and capillary whole blood.


  • Sensitivity: 100%
  • Specificity: 100%
  • Storage at 4-30°C
  • Rapid Result time – 15 to 25 Minutes.
  • Detects antibodies specific to HIV and Syphilis.
  • Specimen Type – finger tip whole blood
  • Easy and convenient testing
  • User friendly, Fast and simple
  • Easy differentiation between HIV and Syphilis infection.
  • No additional Instrument required.

You will receive:

  • 50 x One Step HIV 1+2/Syphilis Combo Card Test pouched
  • 2 x Buffer solution
  • 50 x Blood Lancets
  • 50 x Alcohol swab pads
  • 1 x User manual

Test Procedure:

First, remove the test cassette device from the pouch and place it on a stable flat surface.

  1. Sample collection from the fingertip. Wash your hand with soap and warm water or clean with an alcohol swab and allow to dry.
  2.  Gently massage the hand towards the fingertip of the middle or ring finger without touching the puncher site
  3.  Puncture/prick the fingertip with a sterile lancet
  4.  Wipe away the first sign of blood
  5.  Gently rub the hand from wrist to palm to finger to form a rounded drop of blood over the puncture site
  6.  Collect the Fingerstick Whole Blood sample by using the dropper or capillary tube (collect blood up to the mark on the dropper)
  7.  Add 1 drop of the blood vertically to the sample well/hole in the area of the cassette by squeezing the dropper or bulb of the capillary tube.
  8.  Add 2 drops of buffer solution into the same well/hole of the cassette with the blood
  9. Wait for 15 minutes and read the results immediately (do not read the results after 25 minutes)

Test results illustration

Test interpretation:

(Note that the coloured control line (C) must always appear to show that your test is valid).
POSITIVE: Two lines appear. One coloured line should be in the control line region (C) and another
apparent coloured line should be in any of the test line region for HIV and SYP indicating either HIV or Syphilis positive. Note that, any shade of colour in the test line region should be considered positive. Three colour lines appear. One colour should be in the control region (C) and two apparent colours should appear separately in the regions indicating both HIV and Syphilis positive.
NEGATIVE: One coloured line appears in the control line region (C). No line appears in the test line regions for HIV and SYP.
INVALID: Control line fails to appear. Insufficient sample volume or incorrect procedural techniques
are the most likely reasons for control line failure.