POTASSIUM REAGENTS

POTASSIUM REAGENTS

Enzymatic method
FIXED TIME

PRINCIPLE

Potassium measurements are used to monitor electrolyte balance in the diagnosis and treatment of disease conditions characterized by low or high blood potassium levels.

Potassium is  determined spectrophotometrically through a kinetic coupling assay system using potassium dependent pyruvate kinase. Pyruvate generated is converted to lactate accompanying conversion of  NADH to NAD.

The corresponding decrease of optical density at 380 nm is proportional to the potassium concentration in the serum.

REAGENT COMPOSITION
R1 LDH <50 KU/L, NADH <10 mmol/L, sodium azide 0,05%
and stabilizers.
R2 Pyruvate kinase <50 KU/L, sodium azide 0,05% and
stabilizers.
CAL Sodium / Potassium standard. Sodium (Na+) 160 mmol/L/ Potassium (K+) 6.0 mmol/L.
Warning: The reagents in this kit contain sodium azide. Do not allow to contact with skin or mucous membranes.
STORAGE AND STABILITY
Store at 2-8ºC. The Reagents are stable until the expiry date stated on the label.

REAGENT PREPARATION
Ready-to-use.
SAMPLES
Non-hemolysed serum is recommended. Stable for at least 5 days
at 2-8°C. Blood specimens should also be separated from the red
cells shortly after collection to prevent any leakage of potassium
from the intracellular into the extracellular. Specimens for serum
potassium analysis should be free from hemolysis since the high
concentration of potassium released from red cells significantly
increase the serum levels and this invalidates the test results.
Plasma from anticoagulants not containing potassium is also
suitable.

MATERIALS REQUIRED

• Photometer or spectrophotometer with a thermostatic cell compartment set at 37ºC, capable of reading at 380-405 nm.
• Pipettes to measure reagent and samples.

POTASSIUM REAGENTS per box