Albumin Clinical Chemistry Reagents

Albumin Clinical Chemistry Reagents

Colorimetric method
ENDPOINT

PRINCIPLE
The method is based on the specific binding of bromocresol green (BCG), an anionic dye, and the protein at acid pH with the resulting shift in the absorption wavelength of the complex. The  intensity of the color formed is proportional to the concentration of albumin in the sample.

REAGENT COMPOSITION

R1 Bromocresol reagen: . Succinate buffer 75 mmol/L pH 4.3,
BCG 0.12 mmol/L, tensioactive 2 g/L (w/v). 4 x 100ml
CAL Albumin standard: Bovine serum albumin 5 g/dL (50 g/L)
Concentration value is traceable to Standard Reference
Material 927c. 1 x 3ml

STORAGE AND STABILITY

Store at 2-8ºC

REAGENT PREPARATION
The Reagent and Standard are ready-to-use.
SAMPLES
Serum, EDTA plasma unhemolyzed.
Albumin in serum and plasma is stable for 2 weeks at 2-8ºC, and
for up to 4 months at –20ºC

• MATERIALS REQUIRED
• Photometer or colorimeter capable of measuring absorbance at
  630 ± 20 nm.
• Pipettes to measure reagent and samples.
• Timer. This is not necessary if the assay is performed in an
  automated instrument.

Albumin Clinical Chemistry Reagents